SYSTAT 3d directionality plots

<t>3D</t> <t>Directionality</t> analysis of the stromal collagen organization with development. Collagen alignment in the developing corneal stroma was quantified by the Directionality plugin in Image J. The percentage of signal/collagen aligned at each angle from −90° to +90° within the image was evaluated and plotted against corneal stromal depth. At E9, the peaks show a preferred alignment of collagen at −90°, 0° and +90°, indicating an orthogonal/non-rotational collagen organization with respect to the choroid fissure (0°). At E10 onwards, the peaks display a shift from 0° illustrating the rotation of collagen at the anterior stroma. In the posterior stroma no shift in the peaks of the preferred collagen alignment is observed, illustrating that there is no rotation in the posterior stroma. Representative data from three samples for each embryonic stage.

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3D Directionality analysis of the stromal collagen organization with development. Collagen alignment in the developing corneal stroma was quantified by the Directionality plugin in Image J. The percentage of signal/collagen aligned at each angle from −90° to +90° within the image was evaluated and plotted against corneal stromal depth. At E9, the peaks show a preferred alignment of collagen at −90°, 0° and +90°, indicating an orthogonal/non-rotational collagen organization with respect to the choroid fissure (0°). At E10 onwards, the peaks display a shift from 0° illustrating the rotation of collagen at the anterior stroma. In the posterior stroma no shift in the peaks of the preferred collagen alignment is observed, illustrating that there is no rotation in the posterior stroma. Representative data from three samples for each embryonic stage.

Journal: Acta biomaterialia

Article Title: Cell regulation of collagen fibril macrostructure during corneal morphogenesis

doi: 10.1016/j.actbio.2018.08.017

Figure Lengend Snippet: 3D Directionality analysis of the stromal collagen organization with development. Collagen alignment in the developing corneal stroma was quantified by the Directionality plugin in Image J. The percentage of signal/collagen aligned at each angle from −90° to +90° within the image was evaluated and plotted against corneal stromal depth. At E9, the peaks show a preferred alignment of collagen at −90°, 0° and +90°, indicating an orthogonal/non-rotational collagen organization with respect to the choroid fissure (0°). At E10 onwards, the peaks display a shift from 0° illustrating the rotation of collagen at the anterior stroma. In the posterior stroma no shift in the peaks of the preferred collagen alignment is observed, illustrating that there is no rotation in the posterior stroma. Representative data from three samples for each embryonic stage.

Article Snippet: 3D directionality plots were then generated using SigmaPlot13 (Systat Software, San Jose, CA) to show the collagen/keratocyte alignment across the corneal stromal depth with development.

Techniques:

3D Directionality analysis of the keratocyte organization in the corneal stroma with development. Directionality analysis, representing the percentage of keratocytes aligned along a particular direction (from −90° to +90°) against corneal stromal depth. At E8, keratocytes are predominantly orientated randomly along the choroid fissure axis (0°), as shown by the appearance of various peaks. At E9, peaks appear at −90°, 0° and +90°, indicating that the cells take up a more orthogonal orientation. Also, there is a shift in the peaks from 0° in the anterior stroma illustrating rotation of the keratocytes in the anterior stroma. There is an increase in the shift of the peaks with development, indicating an increase in the keratocyte angular displacement through the anterior corneal stromal depth as the cornea develops. In the posterior stroma the keratocytes are orthogonally organized but no rotation is evident. Representative data from three samples for each embryonic day.”

Journal: Acta biomaterialia

Article Title: Cell regulation of collagen fibril macrostructure during corneal morphogenesis

doi: 10.1016/j.actbio.2018.08.017

Figure Lengend Snippet: 3D Directionality analysis of the keratocyte organization in the corneal stroma with development. Directionality analysis, representing the percentage of keratocytes aligned along a particular direction (from −90° to +90°) against corneal stromal depth. At E8, keratocytes are predominantly orientated randomly along the choroid fissure axis (0°), as shown by the appearance of various peaks. At E9, peaks appear at −90°, 0° and +90°, indicating that the cells take up a more orthogonal orientation. Also, there is a shift in the peaks from 0° in the anterior stroma illustrating rotation of the keratocytes in the anterior stroma. There is an increase in the shift of the peaks with development, indicating an increase in the keratocyte angular displacement through the anterior corneal stromal depth as the cornea develops. In the posterior stroma the keratocytes are orthogonally organized but no rotation is evident. Representative data from three samples for each embryonic day.”

Techniques:

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